ESR Spectrometry

Electron Spin Resonance (ESR) spectrometry is an analytical method to observe and measure absorbed energy on irradiation of the microwave to the radicals in a strong magnetic field. Recently, the function of active oxygen species, such as the hydroxyl radical and super oxide in vivo, have attracted attention. These radicals can be studied by the ESR spectrometry. Generally, these radicals are unstable, but when they are first reacted with a spin trapping reagent, they can be trapped and stabilized. On the other hand, with use of a spin labeling reagent, the molecules without radicals can also be measured with ESR.

Spin Trapping Reagents

Spin Labels

Spin Trapping Reagents

T1534 T1534 M1164 M1164 T1554 T1554
D2362 D2362 D3068 D3068 B1735 B1735
B1701 B1701 N0367 N0367
T1534 2,4,6-Tri-tert-butylnitrosobenzene
M1164 2-Methyl-2-nitrosopropane Dimer
T1554 3,3,5,5-Tetramethyl-1-pyrroline N-Oxide
D2362 5,5-Dimethyl-1-pyrroline N-Oxide
D3068 5-(Diethylphosphono)-5-methyl-1-pyrroline N-Oxide
B1735 N-tert-Butyl-alpha-(4-pyridyl-1-oxide)nitrone
B1701 N-tert-Butyl-alpha-phenylnitrone
N0367 Nitrosobenzene
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Spin Labels

D2399 D2399 T1560 T1560 C1406 C1406
C1432 C1432 I0487 I0487 A1348 A1348
A1343 A1343 C1428 C1428 C1782 C1782
H0865 H0865 H0878 H0878 I0486 I0486
M1197 M1197 O0266 O0266
D2399 16-DOXYL-stearic Acid Free Radical
T1560 2,2,6,6-Tetramethylpiperidine 1-Oxyl Free Radical
C1406 3-Carboxy-2,2,5,5-tetramethylpyrrolidine 1-Oxyl Free Radical
C1432 4-(2-Chloroacetamido)-2,2,6,6-tetramethylpiperidine 1-Oxyl Free Radical
I0487 4-(2-Iodoacetamido)-2,2,6,6-tetramethylpiperidine 1-Oxyl Free Radical
A1348 4-Acetamido-2,2,6,6-tetramethylpiperidine 1-Oxyl Free Radical [Catalyst for Oxidation]
A1343 4-Amino-2,2,6,6-tetramethylpiperidine 1-Oxyl Free Radical
C1428 4-Carboxy-2,2,6,6-tetramethylpiperidine 1-Oxyl Free Radical
C1782 4-Cyano-2,2,6,6-tetramethylpiperidine 1-Oxyl Free Radical
H0865 4-Hydroxy-2,2,6,6-tetramethylpiperidine 1-Oxyl Free Radical
H0878 4-Hydroxy-2,2,6,6-tetramethylpiperidine 1-Oxyl Benzoate Free Radical [Catalyst for Oxidation]
I0486 4-Isothiocyanato-2,2,6,6-tetramethylpiperidine 1-Oxyl Free Radical
M1197 4-Methoxy-2,2,6,6-tetramethylpiperidine 1-Oxyl Free Radical [Catalyst for Oxidation]
O0266 4-Oxo-2,2,6,6-tetramethylpiperidine 1-Oxyl Free Radical
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